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ARTICLE
A FATAL CASE OF PNEUMOCEPHALUS LINKED TO HYPERMUCOVISCOUS, HYPRECLINICAL SAFETY ASSESSMENT OF EUPHORBIA HIRTA EXTRACTS: ACUTE AND SUBACUTE TOXICITY IN RODENT MODELS
Issue Vol. 1 No. 01 (2024): Volume 01 Issue 01 --- Section Articles
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Abstract
Euphorbia hirta, a widely used medicinal plant, holds significant traditional and pharmacological importance. This study aimed to evaluate the acute and subacute oral toxicity of Euphorbia hirta methanol extract in mice and Wistar rats, respectively. In the acute toxicity study, single oral doses up to 5000 mg/kg in mice caused no mortality or significant clinical signs, indicating a low acute toxicity profile (LD50 > 5000 mg/kg). For the subacute study, Wistar rats received daily oral doses of 250, 500, or 1000 mg/kg for 28 days. No significant changes were observed in body weight, organ weights, food/water intake, hematological parameters (RBC, WBC, Hb, PCV, platelets), or biochemical markers of liver (ALT, AST, ALP) and kidney (creatinine, urea) function across all treated groups compared to controls. Histopathological examination of vital organs (liver, kidneys, spleen, heart, lungs, brain) also revealed no morphological alterations. These findings suggest that Euphorbia hirta methanol extract has a favorable safety profile with no significant systemic or organ-specific toxicity at the tested doses, supporting its traditional use and potential for therapeutic development.
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