ENHANCING MAXILLOFACIAL BONE REGENERATION: AN IN VIVO CANINE MODEL ASSESSMENT OF NANOSTRUCTURED IRON-MANGANESE ALLOYS DOPED WITH COPPER, TUNGSTEN, AND COBALT

Dr. Nelira D. Vossan; Dr. Terun A. Clevoy; Dr. Vimlesh K. Dornet

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ARTICLE

ENHANCING MAXILLOFACIAL BONE REGENERATION: AN IN VIVO CANINE MODEL ASSESSMENT OF NANOSTRUCTURED IRON-MANGANESE ALLOYS DOPED WITH COPPER, TUNGSTEN, AND COBALT

Dr. Nelira D. Vossan ¹ , Dr. Terun A. Clevoy ² , Dr. Vimlesh K. Dornet ³

¹ Department of Biomedical Engineering, University of Malaya, Kuala Lumpur, Malaysia

² Faculty of Dentistry, Cairo University, Egypt

³ Centre for Biomaterials and Tissue Engineering, Indian Institute of Technology (IIT) Kanpur, India

Issue Vol. 1 No. 01 (2024): Volume 01 Issue 01 --- Section Articles --- Published Date: 2024-12-08

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Abstract

The development of biodegradable metallic alloys for medical applications, particularly in maxillofacial and orthopedic surgery, represents a significant leap forward in implantology. These materials offer the promise of providing robust mechanical support during tissue healing while gradually degrading, thereby obviating the need for secondary removal surgeries {2, 45}. This investigation delves into the osteogenic capabilities of novel nanostructured iron-manganese (Fe-Mn) based alloys individually doped with copper (Cu), tungsten (W), and cobalt (Co). The study was conducted using a rigorous in vivo canine model to closely mimic human physiological conditions. Four distinct alloy compositions were fabricated using advanced mechanical alloying and selective laser melting techniques: a baseline FeMn35 alloy (M0), and three variants, FeMn32Cu3 (M1), FeMn32W3 (M2), and FeMn32Co3 (M3). These alloys were implanted into surgically created critical-sized mandibular defects in ten mongrel dogs, with an empty defect group serving as a control (M). After a 12-week healing period, a comprehensive multi-modal analysis was performed, including cone-beam computed tomography (CBCT), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX), detailed histological and histomorphometric evaluations, and quantitative real-time polymerase chain reaction (qRT-PCR) for key osteogenic gene markers (osteopontin and osteocalcin). The results demonstrated that all alloy-implanted defects exhibited enhanced bone formation compared to the control group, which primarily formed immature woven bone. Quantitative analysis revealed a clear hierarchy of osteogenic potential among the alloys. The cobalt-doped alloy (M3) showed a statistically significant superior performance across all metrics, including the highest percentage of new bone area, the greatest degree of bone maturation, and the most pronounced upregulation of osteopontin and osteocalcin expression. The tungsten-doped alloy (M2) and copper-doped alloy (M1) also showed significant improvements over the baseline Fe-Mn alloy and the control group. SEM-EDX analysis post-implantation confirmed the onset of biodegradation and the deposition of calcium and phosphorus on the alloy surfaces, indicating active biomineralization. The collective findings strongly suggest that the incorporation of cobalt, tungsten, and copper into a nanostructured Fe-Mn matrix significantly enhances its biocompatibility and osteogenic potential {18}. These results underscore the promise of these novel biodegradable alloys for clinical use in maxillofacial reconstruction and other load-bearing orthopedic applications, paving the way for improved patient outcomes and reduced healthcare burdens {19}.

Keywords

Biodegradable Metals, Iron-Manganese Alloys, Maxillofacial Surgery, Osteogenesis

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ENHANCING MAXILLOFACIAL BONE REGENERATION: AN IN VIVO CANINE MODEL ASSESSMENT OF NANOSTRUCTURED IRON-MANGANESE ALLOYS DOPED WITH COPPER, TUNGSTEN, AND COBALT. (2024). European Journal of Emerging Medicine and Public Health, 1(01), 6-24. https://www.parthenonfrontiers.com/index.php/ejemph/article/view/174

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ENHANCING MAXILLOFACIAL BONE REGENERATION: AN IN VIVO CANINE MODEL ASSESSMENT OF NANOSTRUCTURED IRON-MANGANESE ALLOYS DOPED WITH COPPER, TUNGSTEN, AND COBALT

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